Figure 5.

Pamidronate and Zoledronate induce S-phase arrest in MCF-7 cells. Tumor cells were either left untreated (A) or pretreated with 100 µM Pamidronate (B) or 100 µM Zoledronate (C) for 16-18 hours. Cells were fixed in chilled 70% ethanol and stained with 5 µg/ml of propidium iodide. Ten thousand events were acquired on a FACS Calibur and cell cycle analysis was performed. Data representative of two independent experiments are shown.