Figure 7.

Cytotoxicity of Vγ9Vδ2 T cells against Pamidronate- and Zoledronate-treated MCF-7 is mediated by γδ TCR and NKG2D. Pamidronate- (A) and Zoledronate (B)-treated MCF-7 cells were incubated with Vγ9Vδ2 T cells at an E:T ratio of 30:1 and the percentage of specific lysis determined by ⁵¹Cr release assay. Vγ9Vδ2 T cells were untreated (Unt) or treated with anti-γδ TCR (10 µg/ml), anti-Vγ9 (10 µg/ml) and anti-NKG2D (20 µg/ml) mAb or a combination of anti-γδ TCR (10 µg/ml) and anti-NKG2D (20 µg/ml) or anti-Vγ9 (10 µg/ml) and anti-NKG2D (20 µg/ml) mAbs. As control, Vγ9Vδ2 T cells treated with isotype specific antibodies were used as effectors and co-cultured with tumor targets. Error bars indicate % cytotoxicity±SE. Statistical significance: #, P < 0.002; @, P < 0.003; *, P < 0.0001. Data is representative of two independent experiments.