Figure 8.

Cytotoxicity of Vγ9Vδ2 T cells against Pamidronate- and Zoledronate-treated MCF-7 cells is partially dependent on the perforin-granzyme pathway. Pamidronate- (A) and Zoledronate (B)-treated MCF-7 cells were incubated with Vγ9Vδ2 T cells at an E:T ratio of 30:1 and the percentage of specific lysis was determined by a 4-hour ⁵¹Cr release assay. Vγ9Vδ2 T cells were either untreated or pretreated with concanamycin A (200 nM) for 1 hour at 37˚C and then added to targets and further incubated for 4 hours at 37˚C. Error bars indicate % cytotoxicity±SE. Statistical significance: *, P < 0.001; #, P < 0.05. Shown are means of two independent experiments.