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. Author manuscript; available in PMC: 2010 Dec 9.
Published in final edited form as: Dev Dyn. 2008 May;237(5):1243–1254. doi: 10.1002/dvdy.21517

Figure 1. FGF signaling is necessary for initiation of xbra expression.

Figure 1

All embryos were processed by RNA in situ hybridization for expression of xbra. Embryos were treated with SU5402 (90–100 uM) (A–D, K–P) or DMSO alone (E–J). (A–D) The bar represents time from fertilization until eleven hours post-fertilization with each hour represented by hash marks; the black region represents the time the embryos were in carrier (DMSO) only, and the blue region represents the time frame when embryos were treated with the inhibitor SU5402. (A–D) lateral views. (A) DMSO only; (B,C) initially treated with DMSO, then SU5402 was added at 10 and 9 hours post-fertilization, respectively. (D) Embryos were first treated with SU5402 then the inhibitor was washed out after 4 hours. (E–P) Embryos were treated with either DMSO alone or SU5402 from the 8–16-cell stage until the indicated time point post-fertilization and xbra expression was analyzed in these blastula stage embryos. Embryos were cleared in BB:BA (2:1). The two views in each panel are of the same embryo with an animal pole view above and a lateral view below.