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. 2010 Nov 3;11(11):4361–4380. doi: 10.3390/ijms11114361

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© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 6. — Effects of 2-BP on viability of the mouse embryonic stem cell line, ESC-B5. ESC-B5 cells were treated with the indicated doses of 2-BP for 24 h or left untreated. Cell viability was determined with the MTT assay (A) and apoptosis detected using ELISA (B). (C) Percentages of apoptosis and necrosis were determined by staining cells with propidium iodide and Hoechst 33342. (D) Activity of LDH released in the culture medium of ESC-B5 cells after treatment with various concentrations of 2-BP. Data are expressed as a percentage of the maximal level (Max) of LDH activity determined after total cell lysis. Values are presented as means ± SEM of six determinations. ***P < 0.001 versus control (without 2-BP treatment) group.