Figure 7.

Effects of 2-BP on embryo development, assessed using the embryoid body formation assay. ESC-B5 cells were incubated with the indicated concentrations of 2-BP for 24 h or left untreated. (A) Cells were dissociated with trypsin-EDTA, and cultured in cell medium without LIF to induce differentiation. Embryoid bodies were formed with the hanging method, as described in Materials and Methods. (B) Cell extracts (60 μg) were immunoblotted with anti-p-STAT3 or OCT4 antibodies. (C) ESC-B5 cells were incubated with the indicated concentrations of 2-BP for 24 h or left untreated. Cells were further treated with 50 ng/mL nerve growth factor (NGF) for 14 days. Cell extracts (60 μg) were immunoblotted with anti-microtubule associated protein 2 (MAP-2) antibody. Values are presented as means ± SEM. ***P < 0.001 versus the control group.