RIA |
standard curves obtained using phosphate buffer 0.025 mol/L, pH 7.5, more similar to EBC matrix |
10 pg/mL |
[91] |
samples stored at −80 °C before analysis |
10 pg/mL |
[36] |
EIA |
after sampling no further preparation of the sample is necessary, samples stored at −80 °C before analysis |
4–5 g/mL |
[25,46,63, 92–95] |
SPE of pooleed sample (35.5 mL), HPLC purification |
10 pg/mL |
[90] |
LC-MS/MS |
samples pretreated with 50 μL of immunoaffinity sorbent for 60 min |
1 pg/mL |
[17] |
after collection sample spiked with 8-isoPGF2α-d4, stored at −80 °C, lyophilization, redissolved in 50 μL of water/methanol (1:1) |
8 pg/mL |
[85] |
sample spiked with 8-isoPGF2α-d4, pretreated with 50 μL of immunoaffinity sorbent for 60min at 20 °C, centrifuged (3000 rpm for 5 min), immunoaffinity sorbent flushed twice with water, elution with methanol, methanol evaporated, redissolved in 50 μL of mobile phase |
1 pg/mL |
[14] |
LC-MS |
immunoaffinity separation |
1 pg/mL |
[16] |