Table 3.

Chromatographic parameters in the analysis of biological samples for isoprostanes’ determination.

Specimen	Determined Analyte	Column	Eluents	Ref.
EBC	8-iso-PGF2α	Hypercarb Thermo 100 mm × 2.1 mm × 5 μm	A: acetonitrile B: water (pH 11) isocratic elution A:B (7:3) flow rate 250 μL/min	[14,17]
EBC	8-iso-PGF2α o-Tyr 8-OHdG	Hypercarb Thermo 100 mm × 2.1 mm × 5 μm	A: aqueous solution of ammonium hydroxide pH 10.5 B: methanol:acetonitrile 60:40 (v/v) + 0.1% of ammonium hydroxide gradient elution	[85]
Urine Plasma	8-iso-PGF2α	Symmetry C8 150 mm × 3.9 mm × 5 μm	A: 0.1% acetic acid (pH 3) B: acetonitrile isocratic elution A:B (7:3)	[8]
Urine	8-iso-PGF2α	YMC ODS-AQ 50 mm × 2.0 mm × 3 μm	A: methanol: acetonitrile 5:95(v/v) B: 2 mM ammonium acetate gradient elution flow rate 0.2 mL/min	[7]
Urine	8-iso-PGF2α	Phenomenex Gemini C 18 110 Å, 50mm × 2.0 mm × 3 μm	A: 0.1% aqueous solution of ammonium hydroxide B: 0.1% ammonium hydroxide in acetonitrile gradient elution	[9]
Plasma	8-iso-15(R)PGF2α 11β-PGF2α 15(R)-PGF2α PGF2α	Synergi Hydro-RP 250 mm × 2.0mm	A: water + 0.01% acetic acid B: methanol gradient elution	[10]
Urine	8-iso-PGF2α 8-iso-15(R)-PGF2α 8-iso-PGF2ß PGF2α	Hypercarb, 5 μm, 1 mm × 150 mm, porous graphitic carbon column	A: water + 0.5% aqueous solution of ammonium hydroxide (pH 9.5) B: acetonitrile:methanol 40:60(v/v) + 0.5% aqueous solution of ammonium hydroxide flow rate 50 μL/min gradient elution	[6]