Table 2. Primer sequence for ND and FZD4 gene amplification.

Name	Primer sequence (5′> 3′)	Product size (bp)
NDP-1	F: CGCCTGATTGATATATGACTGCAATGGC	322
	R: GCTCGGTTTGGAAAGAAGCGATTTCCT
NDP-2	F: TTCTGGGTAAATAATTCTGGGG	471
	R: GTTTCTGAGGGAAATGCTCTCCTCACA
NDP-3	F: TAAGGTTGTGGCATGCCCACAGAGTAA	690
	R: CAGAAGATGTCCCAGGAAAAGCTGGGCTTT
FZD4-1A	F: ATAATTTTAGCGCCGCGAGCCTCCAG	771
	R: GAAATCACTTTTCCAGGAGAGCTGTCTCC
FZD4-1B	F: CAAACTGGGGGTGTCTGCCAGAGCA	475
	R: GAAATCACTTTTCCAGGAGAGCTGTCTCC
FZD4-2A	F: GGGAGCATTTGGTCAAACTTCCAAGTC	757
	R: GAGTGTCAGAATAACCCACCAAATGGAGCT
FZD4-2B	F: CCTGTTCTCATCCAAGAAGGACTTAAGAA	781
	R: TTCAAAATGAAGAAAGCATGGAGGCTGACT