Table 3. Primer Sequence for LRP5 gene amplification.
Name | Forward sequence (5′→3′) | Reverse Sequence (5′→3′) | Product size (bp) |
---|---|---|---|
Exon 1 |
TCCTCCCCGTCGTCCTG |
ATTGTCCGAGCAACCCG |
269 |
Exon 2 |
CTTAGCCAGTGGCCCTCA |
AGAGAGAGATGGTGACACT |
496 |
Exon 3 |
TCTGTGTTAGCTGCTTCTCTT |
CCAGGACTGCGTGGGTA |
259 |
Exon 4** |
GATGGCTCCTCCACCCCGCT |
GCGCCCCAGCCGGCACT |
250 |
Exon 5** |
CTCATTCAGAAACAAGTGACGGTCCTC |
GTCCCGTCCCACCGCCT |
216 |
Exon 6 |
CTGCTGCAGGCCCTTGA |
TCTCCCTCTCGCCTGTG |
506 |
Exon 7 |
GTCATGGACTTCTGCTTCTT |
TGGCCTCCTGGATCAAAC |
235 |
Exon 8 |
TGGCCCATCCAGACCTAT |
CAAGTCTGCATGGCTGAG |
298 |
Exon 9 |
GCATTCATTGTGTGGCTTG |
AAGCCTTTGAGGCAGGA |
446 |
Exon 10 |
CTTTTCCTCCTCACCTGCT |
GGTGAACACAAGGACGC |
289 |
Exon 11 |
AGACTCACTGAGCCTGC |
GCCCTCCATGACCAGAAG |
261 |
Exon 12 |
CCTTTGCTGACACCGTG |
GAAGCTCCTTTCAGCGT |
440 |
Exon 13 |
CCTGCAGCCCTGTCTTT |
GCCTTGGGAAGCACACC |
271 |
Exon 14 |
CTCAGGAGTCTTGGTTTCTTT |
GCATTCGGCAGAAGACAC |
308 |
Exon 15 |
CCCACACCCGTCCTTCA |
GGGTGTCTGCGGTTAGG |
263 |
Exon 16 |
GAGGTCAGCACTGCTCA |
GGTCGGGTTTAGAGGCCA |
301 |
Exon 17 |
AGAGCCTGACCTCTGTTT |
TACCTGTCCATCACCCCAA |
206 |
Exon 18 |
GGCTGCGTGTGATGTTC |
GGTCTTGGCAGAGCCTTGA |
307 |
Exon 19 |
AGCCTCTCTGAGTGCAT |
TAAACTCCACGTTCCTGGG |
208 |
Exon 20 |
GGCCACCTCTTTCTGTTT |
AGATCATTCCATATCTCAGGCTC |
294 |
Exon 21** |
GAGTCTCGTGGGTAGTGGGA |
AGAAAGCAAGGCATGCCTCAGAG |
245 |
Exon 22 |
CTGGCGAGGCTCTAAGT |
CCCAATGGCCATGGAGG |
174 |
Exon 23A* |
CTCCTCTGTGTGTGTCCC |
TCAGGATGAGTCCGTGC |
286 |
Exon 23B* | CTACTTCCATCTCTTCCCGC | CGAAAGAATGGCAGTTCTGTT | 271 |
Primer sequences obtained from Gong et al. [16] ** Exon 23 was amplified in two overlapping segments, A and B.