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Glucosamine (GlcN) suppressed epidermal growth factor (EGF)-induced increases in β-catenin. The human retinal pigment epithelial cell line (ARPE-19) cells were cultured to 80% confluence, treated with 2.5 mM or 5 mM GlcN in serum-free medium for 24 h, then stimulated with EGF for further 24 h and probed with antibody against β-catenin.
