Fig. 2.

ISH analysis of i17a-containing BKCa channel mRNAs in hippocampal neurons. (A and B) Photomicrographs showing ISH of cultured hippocampal neurons with i17a antisense (A) and i17a sense (B) probes (n = 3). (Insets) Photomicrograph MAP-2–positive regions for each. Biotin-labeled oligo probes (30 nucleotides long) were used for detection. Qdot 605 streptavidin conjugates were used for visualization. (Scale bars: 20 μm.) (C) Schematic showing the STREX region BKCa channel mRNA targets for each siRNA treatment. (D) Summary plot of the average fluorescence (%) for an i17a ISH signal for the indicated condition: si16, 100 ± 3.97, n = 9; si17a, 34.96 ± 9.73, n = 10; siSTX, 35.05 ± 6.52, n = 10. Data are mean ± SEM. (Scale bar: 25 μm.)