Fig. 5.

Negative-function assays for Asp-400 substitution mutants. (A) In vivo Φ(narG-lacZ) expression in a narL505 strain, as described for Fig. 3. (B) In vitro transmitter phosphatase activities, as described for Fig. 3. □, no sensor; ▲, D400A; △, D400S; ▼, D400E; ■, D400N; ○, wild type.