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Negative-function assays for Asp-400 and Gln-404 plus H399Q double-substitution mutants. (A) In vivo Φ(narG-lacZ) expression in a narL505 strain, as described for Fig. 3. Mutants carrying the H399Q substitution are indicated by plus sign (“+”). (B) In vitro transmitter phosphatase activities, as described for Fig. 3. □, no sensor; ▽, Q404A+H399Q; ◇, Q404H+H399Q; △, D400A+H399Q; ◆, H399Q; ■, D400N+H399Q; ○, wild type.
