Fig. 1.

D. virilis TART transcripts have 5′ Tags. (A) Genomic DNA with two complete and one 5′-truncated element. Transcription of the central element from the −55 start site in the upstream element produces a transposition intermediate RNA with a new Tag on the 5′ end. Light gray: 5′ and 3′ UTRs. Dark gray triangle: 3′ end of element from −55 through oligoA tail (sequence at bottom of figure). This is added to the new transcript as a Tag and forms the end of the chromosome after reverse transcription. Thin black triangles: partially eroded Tags from earlier transpositions (sequences shown in box). Tag strings are found on 5′ ends of complete, but not 5′-truncated, elements. (B) Organization of partially eroded Tags into strings found on individual elements. “R” denotes Tag strings sequenced from RACE products. “G” denotes strings from genomic DNA. For each element, Tags are ordered with most distal on the left. Each element has several Tags showing multiple transpositions. Tag lengths show variable erosion in successive transpositions.