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AH2 is an ATP-dependent annealing helicase. (A) Schematic diagram of the annealing helicase assay. (B) AH2 catalyzes the rewinding of DNA in an ATP-dependent manner. Annealing helicase assays were carried out in the presence or absence of the indicated factors, and the resulting DNA species were resolved by agarose gel electrophoresis. An equimolar concentration of UTP was used as a control for the absence of ATP.
