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. 2010 Nov 17;107(49):21164–21169. doi: 10.1073/pnas.1010011107

Fig. 3.

Fig. 3.

Wnt-5a ligand alters fEPSP in a concentration- and calcium-dependent manner. (A) Hippocampal slices were exposed to different dilutions of Wnt-5a–conditioned medium. Plot of fEPSP amplitude versus the concentrations of Wnt5a in the medium. Inset shows an immunodetection of Wnt-5a in increasing volumes of Wnt-5a–conditioned medium and control-conditioned medium (C media). rWnt-5a was used as control. (B) Plot of fEPSP amplitude changes with treatment with 2 pM Wnt-5a for 10 min (shown in the box), in the absence or presence of sFRP (25 nM). (C) Plot of fEPSP amplitude changes with treatment with Wnt-3a for 10 min. (D–F) Plots of fEPSP amplitude changes with treatment with 2.0 pM Wnt-5a for 10 min in the absence or presence of zero calcium solution (D), 2 mM cadmium (E), or 5 μM nifedipine (F). (G) Changes in fEPSP amplitude with treatment with control-conditioned medium or 25 nM sFRP. Dots and bars represent means ± SE from six different slices.