Fig. 4.

The effect of RD3 expression on the localization of GC1 in transfected COS-7 cells. Transfected COS-7 cells expressing either RD3 or GC1 alone or coexpressing RD3 with either GC1 or ABCA4 were labeled with antibodies to RD3, GC1, ABCA4, and Rab11 or calnexin as indicated. (A–C) RD3 colocalized with Rab11 to intracellular vesicles of RD3-transfected cells. (D–F) GC1 colocalized with the ER marker calnexin in a pattern characteristic of ER localization in GC1-transfected cells. (G–I) RD3 recruited GC1 out of the ER into distinct vesicles that colabeled with RD3 and GC1 antibodies in cells cotransfected with RD3 and GC1. (J–L) Intracellular vesicles colabeled with RD3 and Rab 11 antibodies in cells coexpressing RD3 and GC1. (M–O) Distinct intracellular vesicles were labeled with RD3 and ABCA4 antibodies in cells coexpressing RD3 and ABCA4. (Scale bar: 20 μm.)