Fig. 4.

Comparison of AurA regulation by Cep192 and TPX2. (A) Quantitative comparison of AurA T295 phosphorylation induced by TPX2-NT and αAurA (see Fig. S7B for the relevant data). (B and C) Intact AurA–Cep192 complexes are essential for the efficient phosphorylation of endogenous maskin in sperm nuclei- or αAurA-supplemented extracts. Mock-treated and Cep192-depleted (B) or naïve (C) egg extracts were incubated with the indicated components and [γ-³²PO₄]ATP. Extracts were diluted with radio-immunoprecipitation assay (RIPA) buffer and subjected to IP with anti-maskin Ab. IPs were analyzed by autoradiography and the results quantified as described in SI Materials and Methods (see Fig. S7 G and H, Upper for the relevant data). The calculated values for the corresponding control extracts were set at 100%. (D) Model of AurA regulation by TPX2 (10–16, 25) and Cep192.