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. 2010 Nov 15;107(49):21028–21033. doi: 10.1073/pnas.1004169107

Fig. 6.

Fig. 6.

Distinct effects of β-blocker drugs on plasma membrane recruitment of hippocalcin. Hippocampal neurons were infected with lentiviral vector encoding hippocalcin-EGFP for 24–48 h before TIRFM imaging. (A) Application of 10 μM norephinephrine decreased hippocalcin fluorescence levels to 89.1 ± 3% relative to basal levels within 5 s after drug addition (n = 6). (B) Application of 10 μM propranolol increased surface hippocalcin fluorescence to 106.5 ± 1.8% of basal levels within 5 s after drug addition (n = 6). (C) Application of 10 μM carvedilol increased surface hippocalcin-EGFP fluorescence to a maximum of 183 ± 12.7% relative to basal levels within 5 s after bath application, and therafter produced a sustained phase of recruitment lasting ∼1 min (n = 6). (D) Bar graph analysis of surface hippocalcin-EGFP fluorescence intensity effects measured across multiple neurons within 5 s after bath application (isoproterenol, 89.1 ± 3%, n = 6; propranolol, 106.5 ± 1.8%, n = 6; carvedilol, 183 ± 12.7%, n = 6). All percentages represent mean fluorescence determinations relative to basal levels.