Fig. 2.

Identification of conserved regions and amino acids within the assembly domain that are important for filament assembly. (A) Structural model of the tail fragment (1744–1968) used for deletion and mutational analyses. Gray bar identifies the assembly domain (1849–1940). Black bar denotes the essential region for filament assembly identified by internal deletion analysis. (B) Quantification of solubility of myosin II tail domains with regions removed. Error bars represent one SD from three independent experiments. (C–E) Quantification of solubility properties of mutation-containing tail fragments organized by location of mutation in distinct tail regions. Error bars represent one SD from three independent measurements.