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. 2010 Dec 9;5(12):e14283. doi: 10.1371/journal.pone.0014283

Figure 3. Comparison of different interspecies recPrP seeding.

Figure 3

Amyloid formation in the seeding assay was monitored using Thioflavin T. The fluorescence signals were recorded every 30 min at 37°C at recPrP amyloid forming conditions. Fluorescence intensities were summarized from 24–48 h (saturation phase). (Left to right) Interspecies transmission: OvPrPSc in SHaPrP(90–231); OvPrPSc in BovPrP(25–241); SHaPrPSc in OvPrP(25–233); SHaPrPSc in BovPrP(25–241); BovPrPSc in OvPrP(25–233). Interspecies barrier: BovPrPSc in SHaPrP(90–231); SHaPrPSc in MuPrP(89–231). As control parallel an approach was made with the corresponding intraspecies transmission (data not shown).