Table 4. Dose dependent effect of primaquine on the development of 9 day hepatocyte stage parasites.

PQ Dose (µg/mL)	Pv Hepatocyte Stage Parasites/Well					Reduction in Liver Stage Parasites (%)
	Well 1	Well 2	Well 3	Mean	STDEV
0.0	521	465	492	492.67	28.01	-
0.001	467	483	512	487.33	22.81	1.20
0.01	416	427	478	440.33	33.08	10.75
0.1	301	253	289	281.00	24.98	43.00
1.0	183	142	163	162.67	20.50	66.90
10.0	71	96	43	70.00	26.51	85.80

50,000 fresh Pv spz were added to wells containing 20,000 hepatoma cells and were cultured for 9 days in medium alone or in the presence of different concentrations of primaquine (PQ). The numbers of late liver stage schizonts (9 day assay) were counted by immunoflourescence microscopy after staining with the anti-PvCSP mAb, NVS3 as described in Materials and Methods.