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. 2010 Dec 9;5(12):e15281. doi: 10.1371/journal.pone.0015281

Figure 6. Presence of Cq-IAG in hAGs from endocrinologically-induced versus dsCq-IAG-injected crayfish.

Figure 6

Immunohistochemistry was performed on sections from the base of the fifth pereiopod of induced (top) and Cq-IAG dsRNA-injected (bottom) intersex animals. Large quantities of Cq-IAG, demonstrated by the green fluorescence of bound goat anti-rabbit FITC conjugated antibodies, were observed in the cytoplasm of induced AG cells (B, C). Reduced levels of the Cq-IAG hormone were observed in the cytoplasm of AG cells of dsCq-IAG -injected intersex animals (F). DAPI counterstain was used to identify nuclei in both induced (A, C) and silenced (D, F) intersex animals. Bar  = 20 µm.