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. 2010 Dec 9;6(12):e1001237. doi: 10.1371/journal.pgen.1001237

Figure 5. Overexpression of Atg1 rescues PINK1 mutant phenotypes.

Figure 5

Overexpression of Atg1 in the muscle of PINK1B9 mutants rescued their abnormal wing posture (A), thoracic indentation (B), jump/flight activity (C) and muscle ATP level (D). Data are presented as mean ± s.e.m. Statistical significance was determined by Student's t test (*P<0.001). (E) Atg1 overexpression did not completely rescue the mitochondrial aggregation phenotype in the muscle of PINK1 RNAi flies. mitoGFP was expressed in the muscle using Mhc-Gal4 driver to visualize mitochondrial morphology by live imaging. Wild type flies showed mitochondria of relatively uniform sizes (bottom right), while PINK1 RNAi flies had bright mitochondrial aggregates. Only the co-expression of Marf RNAi or Parkin OE was able to efficiently rescue the mitochondrial aggregation phenotype in the PINK1 RNAi background.