Figure 2. HDAC3 and HDAC11 also suppress (CGG)₉₀-eGFP mRNA–induced neurodegeneration.

Co-expression of HDAC11, a class IV HDAC and HDAC6 interacting partner, suppresses (CGG)₉₀-eGFP induced neurodegeneration similarly to HDAC6 in either of two HDAC11-expressing lines (C and D compared to A). Overexpression of HDAC11 alone had no phenotype (B). Lowering endogenous dHDAC11 by siRNA significantly enhances (CGG)₉₀-eGFP induced neurodegeneration (2E versus 1E). Similarly, overexpression of HDAC3, a class I HDAC, suppresses the (CGG)₉₀-eGFP phenotype (F). G) Quantitation of the phenotypes in Figure 1 and Figure 2. Analysis of ∼20 flies per genotype demonstrates highly significant alterations in the phenotype. * represents P<0.01 by a Students' unpaired t-test. The lack of error bars on (CGG)₉₀-eGFP line 1 and (CGG)₉₀-eGFP line 2 co-expressed with siRNAs against HDAC6 and 11 reflect the fact that all observed flies had the most severe phenotype score.