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. 2010 Dec 9;6(12):e1001239. doi: 10.1371/journal.pgen.1001239

Figure 3. Intracellular localization of the wild-type ATGL protein and the mutants D166G and D244fs as determined by laser scanning microscopy.

Figure 3

This image shows the intracellular localization of the YFP-tagged ATGL mutants D166G and D244fs compared to the wild type ATGL in Cos-7 cells loaded with 400 µM fat free BSA-complexed oleic acid to induce lipid droplets generation. Lipid droplets were stained using BODIPY 558/568 C12. While the binding to the lipid droplets was still intact and well pronounced for the D166G mutation, the deletion of the C-terminus in the frameshift mutant D244fs effectively abolished the binding of the protein to the lipid droplets. The merged image clearly shows a diffuse YFP signal in the cytoplasm, which no longer co-localizes with the signal of the BODIPY-stained lipid droplets. The images for all assayed mutants are provided in Figures S1 and S2.