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. 2010 Dec 9;6(12):e1001243. doi: 10.1371/journal.pgen.1001243

Figure 2. EpsE is required to produce EPS.

Figure 2

A. Top row contains images of the chambers of a 12-well microtiter dish containing ethanol-precipitated supernatant from the indicated strain. Bottom row contains ethanol precipitated supernatant from the indicated strains resolved in the stacker of an SDS-PAGE gel stained with Stains-All. All strains contain sinR::kan and tasA::Tn10 spec mutations to increase expression of the eps operon and to liberate the EPS from the cell surface, respectively. The indicated wild type and mutant strains are as follows: “WT” (DS991), epsH (DS5187), ΔepsE (DS2369), ΔepsE +epsEWT (DS2370), ΔepsE +epsED94A (DS2372), ΔepsE +epsEG12D (DS6191), ΔepsE +epsED97G (DS6195), ΔepsE +epsED182G (DS6190), and ΔepsE +epsEK106E (DS6312). B. The indicated “WT” (DS991) and epsH (DS5187) supernatants were treated with (+) or without (−) Proteinase K or DNase and RNase, ethanol precipitated, and resolved in the stacker of an SDS-PAGE gel stained with Stains-All. C. Top, wells contain precipitated supernatant. Bottom, stacking gel contains precipitate resolved by SDS-PAGE and stained with Stains-All. The indicated wild type and mutant strains are as follows: “WT” (DS991), ΔepsE (DS2369), and ΔepsE +epsERBS (DS7147).