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. 2010 Dec 9;6(12):e1001243. doi: 10.1371/journal.pgen.1001243

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Guttenplan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) The pellicle images are top views of DS1674 (sinR epsH) and DS4532 (sinR epsH fliG^V338A) grown at 25°C for the indicated time period. B) The graph represents the percentage of isolated colonies from the indicated strains that were non-motile when dilution plated on Day 0 and Day 4. Error bars are the standard deviation of three replicates. C) Fluorescent micrographs of the parent strains and isolates from 4 days of growth stained with FM4-64 (membrane, false colored red) and Alexa Fluor C₅ 488 maleimide (flagella, false color green). All strains express the modified flagellar filament protein Hag^T209C. The following strains were used to generate this figure: sinR epsH (DS2179), sinR epsH fliG^V338A (DS7289), sinR epsH 4 day isolate (DS7290), and sinR epsH fliG^V338A saf non-motile isolate (DS7293). Scale bar equals 2µm.