Table 3.
Comparison of pCS20 PCR, pCS20 real-time PCR, pCS20 LAMP, and sodB LAMP for the detection of E. ruminantium in various field samples
| No. of samples: | ||||||
|---|---|---|---|---|---|---|
| Sample type | Origin (Site/Country) | Tested | pCS20 PCRa positive | pCS20 real-time PCR positive | pCS20 LAMP positive | sodB LAMP positive |
| Bovine blood | Butaleja/Uganda | 50 | 0 | NDb | 0 | 0 |
| Petauke/Zambia | 50 | 0 | ND | 0 | 0 | |
| Serengeti/Tanzania | 50 | 0 | ND | 0 | 0 | |
| Goat blood | Chama/Zambia | 35 | 0 | ND | 0 | 0 |
| Lamb's blood | Kerr Seringe/The Gambia | 19 | 2 | 2 | 2 | 2 |
| Sheep bloodc | NAd | 4 | 4 | 4 | 4 | 4 |
| Tick;Amblyomma variegatum | Amuria/Uganda | 20 (15/5)e | 2 (2/0) | 4 (4/0) | 4 (4/0) | 4 (4/0) |
| Butaleja/Uganda | 20 (18/2) | 0 | 1 (1/0) | 1 (1/0) | 1 (1/0) | |
| Dokolo/Uganda | 20 (12/8) | 1 (1/0) | 2 (2/0) | 1 (1/0) | 1 (1/0) | |
| Kaberamaido/Uganda | 20 (14/6) | 0 | 1 (0/1) | 1 (0/1) | 1 (0/1) | |
| Pallisa/Uganda | 20 (10/10) | 2 (2/0) | 2 (2/0) | 2 (2/0) | 2 (2/0) | |
| Soroti/Uganda | 20 (17/3) | 2 (2/0) | 2 (2/0) | 2 (2/0) | 2 (2/0) | |
| Tororo/Uganda | 20 (10/10) | 1 (1/0) | 1 (1/0) | 1 (1/0) | 1 (1/0) | |
| Subtotal for tick samples | 140 (96/44) | 8 (8/0) | 13 (12/1) | 12 (11/1) | 12 (11/1) | |
aPCR was performed using KAPA Blood PCR kit.
bND, not done.
cBlood samples from sheep experimentally infected with E. ruminantium were used as positive controls.
dNA, not applicable.
eTotal no. of ticks (No. of male ticks/No. of female ticks).