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. 2010 Nov 23;9:302. doi: 10.1186/1476-4598-9-302

Figure 3.

Figure 3

p30 interacts with Cyclin E and CDK2 and reduces formation of the Cyclin E-CDK2 complex. A and B) p30 disrupts interactions between Cyclin E and CDK2. 293T cells were tranfected with Cyclin E-myc, p30-flag and CDK2-HA. The interactions between Cyclin E and CDK2 in the presence or absence of p30 were assessed by either co-IP of Cyclin E or CDK2 and western blot for the corresponding partner. C) Phosphorylation of Rb protein. 293T cells were transfected with empty control vector or p30 expressing vector, synchronized with "HU" and released for 0 h and 8 h. The cells extracts were then analyzed by WB to detect phosphorylated Rb. D) Interactions between Cyclin E and p30 or (E) CDK2 and p30 were investigated after transient transfection in 293T cells and Co-IP/WB.