Fig. 3.

Aminocyclohexanecarboxylic acids (ACHCs) do not interact with PAT2. A. l-[³H]Proline (100 μM) uptake (pH 5.5, Na⁺-free) was measured in the absence (control) and presence of proline (Pro) and various ACHCs (all 10 mM), 1-aminocyclohexanecarboxylic acid (1-ACHC), trans-2-aminocyclohexanecarboxylic acid (T2-ACHC), cis-4-aminocyclohexanecarboxylic acid (C4-ACHC) and cis-2-aminocyclohexanecarboxylic acid (C2-ACHC). Data are mean ± SEM (n = 18–20). ***, p < 0.001; ns, p > 0.05, versus control. B. Representative current traces observed using PAT2 cRNA- or water-injected oocytes during exposure to proline (Pro) and various aminocyclohexanecarboxylic acids (all 10 mM). C. Mean data for part B showing PAT2-specific current change. Data are mean ± SEM (n = 4). ***, p < 0.001; ns, p > 0.05, versus current change in water-injected oocytes.