Figure 2.

Probability density distributions of forces required to dissociate myo1c^IQ-tail from supported lipid membranes containing 2% PtdIns(4,5)P₂ at loading rates of 250 ± 29, 930 ± 120, and 1800 ± 230 pN/s (standard deviation). The corrected distributions in the main panels were obtained by subtracting (inset, bottom) frequency distributions (per contact cycle) obtained in the absence of myo1c^IQ-tail from (inset, top) uncorrected frequency distributions. (Solid lines) Best fits of the distributions to a model for a single transition barrier (Eq. 1). The fitting results are presented in Table 1, and information regarding numbers of cycles and interactions are presented in Table S1. Errors are standard deviations calculated from bootstrap data sets.