Figure 3.

Probability density distribution of forces required to extract a biotinylated-lipid from supported lipid membranes using neutravidin-coated pedestals at a loading rate of 1100 ± 130 pN/s (standard deviation). The corrected distribution was obtained by subtracting (inset, bottom) the frequency distribution acquired in control experiments that did not contain neutravidin from (inset, top) the uncorrected frequency distribution. (Solid lines) Best fit of the distribution to a model for a single transition barrier (Eq. 1). (Dashed line) Best fit of a model that assumes the rupture peaks correspond to single or simultaneous double-bond ruptures (Eq. 5). The fitting results are presented in Table 1. Errors are standard deviations calculated from bootstrap data sets.