Table 1.
Loading rate (pN/s) | k0 (s−1) | dtr (nm) | Faverage (pN) |
---|---|---|---|
Myo1cIQ-tail-2% PtdIns(4,5)P2 dissociation | |||
250 ± 29 | 9.4 ± 2.4 | 2.2 ± 0.35 | 5.6 ± 0.33 |
930 ± 120 | 11 ± 1.8 | 0.64 ± 0.051 | 16 ± 0.43 |
1800 ± 230 | 43 ± 9.6 | 0.61 ± 0.13 | 13 ± 0.89 |
Lipid extraction | |||
1100 ± 130 | 12 ± 3.9(8.7 ± 3.7)∗ | 0.33 ± 0.083(0.45 ± 0.085)∗ | 27 ± 1.6 |
Errors are obtained from a bootstrap analysis.
Parameters from fits assuming that the rupture peaks are due to either a single bond or two independent bonds that fail simultaneously (Eq. 5). The fraction of single bonds is A = 0.6 ± 0.1.