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. 2010 Dec 15;99(12):4047–4055. doi: 10.1016/j.bpj.2010.10.022

Figure 3.

Figure 3

Theoretical and experimental intensity changes of membranes in p- and s-polarized evanescent fields after topology changes. Geometries used for the calculations are a spherical vesicle touching the supported membrane (A i), a fused vesicle with half the membrane surface in the plane of the supported membrane and the other half forming a half-sphere (A ii), and a planar circular membrane patch (A iii). (B) Ratios of integrated intensities originating from a membrane patch (A iii) to those originating from a vesicle of the same surface (A i) (solid lines) and of intensities originating from a hypothetical intermediate state (A ii) to those from the original state (A i) (dotted lines) for p-polarization (marked p) and s-polarization (marked s) for polar dipole angles between 0° and 90°. Experimentally determined intensity changes 4 ms after the onset of fusion are added for three different dipole angles: 48° (Bodipy-PC (♦)), 55° (NBD-DPPE (▴)), and 68° (Rh-DOPE (■)).