Figure 5.

Averaged normalized intensities for the first 32 ms after fusion. One hundred mean Rh-DOPE fluorescence intensity traces recorded with s-polarized (marked s) and p-polarized (marked p) excitation were normalized to the last time point before fusion and averaged. Two time points before and 32 ms after fusion are shown as a function of time. The horizontal lines represent the calculated intensity changes expected if a vesicle containing a fluorophore with a polar angle of 68° becomes a flat membrane patch (solid line) or a half-sphere surrounded by a flat ring (dotted line).