Figure 4.

It Is the Splicing Event Rather than the Intron Sequence that Causes RNAPII to Pause at the 3′SS

(A) Diagram showing BSRibo1 mutant branchsite sequence base paired to WT U2 snRNA (left) and to mutant U2 snRNA (right).

(B) RT-qPCR analysis of BSRibo1 pre-mRNA and spliced mRNA accumulation in the presence of WT (left) and mutant (right) U2.

(C–E) ChIP analysis to detect RNAPII on the BSRibo1 gene using anti-Rpb3p antibodies. Details are as in Figures 1C–1E.

Error bars indicate standard error for qPCR performed in triplicate. See also Figure S3 for more detail and for ChIP analysis of pSer5 and pSer2.