Figure 7.

Phosphorylated RNAPII Accumulates around the 3′ Splice Sites of Endogenous Yeast Intron-Containing Genes

ChIP analysis was performed to detect RNAPII with pSer5 (4H8 antibodies, black) or pSer2 (H5 antibodies, gray) along the lengths of the intron-containing genes ASC1, ACT1, DBP2, and APE2 and the intronless genes ADH1 and FMP27, all of which are constitutively expressed in yeast cells grown under steady-state conditions. Results are presented as the percentage of input relative to total RNAPII. Error bars indicate standard error for qPCR performed for three different cultures, each assayed in triplicate. Numbers indicate the positions of primers used for qPCR relative to the start codon of each open reading frame. Vertical arrows indicate the positions of the 3′SS, and in the line drawings, the thick lines indicate the positions of exons with respect to the PCR amplicons. For total RNAPII (anti-Rpb3p) ChIP data, see also Figure S6.