Table 1.

Results grouped by injection order irrespective of PBS concentration

Time of PBS injection∗	No. 1	No. 2	No. 3
Fascicles
Modulus (GPa)	0.55 ± 14	0.54 ± 14	0.54 ± 15
Relative energy dissipation (%)	24.9 ± 3.8	24.7 ± 2.6	24.5 ± 4.5
Diameter (mm)	0.28 ± 0.16	0.28 ± 0.17	0.28 ± 0.16
Fibrils
Modulus (GPa)	2.89 ± 0.23	2.88 ± 0.23	2.93 ± 0.21
Relative energy dissipation (%)	14.8 ± 3.4	14.3 ± 4.5	13.9 ± 4.4
Height (nm)	167 ± 50	168 ± 47	171 ± 48

Values are mean ± SD. None of the parameters differ significantly between time-points.

^∗Time is shown as the injection order (first, second, or third PBS buffer introduced). Each time-point covers two measurements at each PBS concentration.