TABLE 5 .
Effects of inhibitors on 14C-labeled amino acid uptake by the WT and ALZ42 strains
| Strain and presence of added H2 |
14C-labeled amino acid uptake (cpm [103]/108 cells)a at: |
|
|---|---|---|
| 5 min | 10 min | |
| WT | ||
| None | 29.0 ± 2.0 | 31.0 ± 1.1 |
| CCCP | 0.7 ± 0.5b | 0.3 ± 0.2b |
| Orthovanadate | 14.8 ± 1.2b | 18.7 ± 1.0b |
| ALZ42 (Hyb only) | ||
| None | 14.0 ± 1.6 | 18.2 ± 3.5 |
| CCCP | 0.3 ± 0.1b | 0.04 ± 0.07b |
| Orthovanadate | 5.5 ± 0.8b | 9.5 ± 0.7b |
Values represent 14C-labeled amino acid uptake by 108 cells ± standard deviation (n = 4).
Significantly lower uptake level than without inhibitor (P < 0.005 [Student’s t test]).