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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: FEBS Lett. 2010 Nov 9;584(23):4735–4739. doi: 10.1016/j.febslet.2010.11.004

Fig. 3.

Fig. 3

SMN and SmB′ compete with Ku80 and Ku70 for binding sites on coilin. GST-pulldown assays were conducted with a fixed amount of GST-coilin and SMN or SmB′ and increasing amounts (1X to 9X) of Ku80 or Ku70. Input lanes accounts for 20% of the protein used in the 1X reaction. (A) SMN and Ku80 are competing for binding sites on coilin. The western blot was probed with Ku80, SMN and GST antibodies. (B) Ku80 and SmB′ are competing for binding sites on coilin. The western blot was probed with Ku80, T7 (to detect SmB′) and GST antibodies. (C) Ku70 and SMN compete for binding sites on coilin. The western blot was probed with T7 (to detect Ku70), SMN and GST antibodies. (D) Ku70 and SmB′ compete for binding sites on coilin. The western blot was probed with anti-T7 to detect Ku70, anti-SmB to detect SmB′ and anti-GST to detect GST-coilin.