Cerebral arteries were pressurized to 20 or 80 mmHg and then exposed to diltiazem (30 μm), ryanodine (50 μm) or thapsigargin (200 nm). Vessels were subsequently frozen in acetone and processed for the assessment of MYPT1 phosphorylation at the T697 or T855 site. Representative Western blots are presented in A and B, whereas summary data (n = 4 arterial pairs from 4 animals) can be found in C and D, respectively. Phosphorylated MYPT1 was standardized to actin and then expressed relative to 20 mmHg, 80 mmHg or 80 mmHg + diltiazem. *denotes significant difference from 20 mmHg, 80 mmHg or 80 mmHg + diltiazem.