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. Author manuscript; available in PMC: 2011 Nov 23.
Published in final edited form as: Biochemistry. 2010 Nov 1;49(46):9948–9956. doi: 10.1021/bi101165p

Fig. 2. Kinetics of binding of GST-Obsc6231–6260 and GST-Obsc6316–6345 to MBP-sAnk129–155.

Fig. 2

(A, B) Colored curves show binding of serial diluted concentrations of MBP-sAnk1 starting at 3µM to GST-Obsc6316–6345 (A) and GST-Obsc6231–6260 (B). Black curves are fits based on a 1:1 stoichiometry of binding. (C–E) Bar graphs of values of KD, kon, and koff for GST-Obsc6316–6345 A (red) and GST-Obsc6231–6260 (green). *** denotes p < .05. n = 5 for all values.