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. Author manuscript; available in PMC: 2010 Dec 10.
Published in final edited form as: Am J Physiol Endocrinol Metab. 2006 Feb 7;291(1):E128–E136. doi: 10.1152/ajpendo.00592.2005

Fig. 4.

Fig. 4

Tbx3 short interfering (si)RNA reduces osteoblast cell number but does not induce apoptosis in MC3T3-E1 cells. A: transfection efficiency of MC3T3-E1 cells using Alexa fluorlabeled nonsilencing siRNA (200 nM). Cells were incubated for 6 h, and transfection efficiency was determined by number of nonfluorescent vs. fluorescent cells. About 60–70% of cells were successfully transfected. Each image is representation of 2 replicates. B: effect of Tbx3 siRNA on cell number. siRNA-1 and -2, Tbx3-specific siRNA; siRNA-NC, scrambled negative control siRNA. Total siRNA concentrations for each treatment were 100 nM. Data are expressed as %control (RNAiFect alone) and presented as means ± SE; n = 6/treatment. *P < 0.05 vs. Control. #P < 0.05 vs. siRNA-NC. C: effect of Tbx3 siRNA on apoptosis. Tbx3 siRNA-1 = 100 nM. RNAiFect, transfection reagent. Data are expressed as means ± SE; n = 6/treatment. *P < 0.001 vs. Control.