FIG. 2.

Determination of SB10 integration in multiple T cell clones. (A) Generation of T cell clones by FACS sorting of DsRed⁺ cells derived from SB-transfected PBLs. (B) Flow cytometric analysis of DsRed expression in T cell clones. Nineteen representative clones of 88 are shown. Nomenclature of each T cell clone was assigned as follows: The first number indicates the amount of transposase used for transfection; the second number indicates the plate number; the third number indicates the well position. (C) Genomic PCR analysis of SB10 expression in T cell clones. Seventeen of 88 clones are shown.