Table 1.
Transposon and Transposase Combinations Used for Generation of Transgenic Bulk T Cell Populations
| Bulk T cells | T cell source | Transposon | Transgene(s) | Transposase |
|---|---|---|---|---|
| SB21 | PBLs | pT2/Cag-DsReda | DsRed | SB10 DNA |
| SB81 | PBLs | pT2/Cag-NGCDa | NGCD | SB10 DNA |
| SB82 | PBLs | pT2/Cag-NGCDa | NGCD | SB10 DNA |
| AP1 | PBLs | pT2/EF1-fLuc_PGK-GFP:Zeo | GFP-Zeo fusion/fLuc | SB10 DNA |
| AP2 | PBLs | pT2/EF1- fLuc_PGK-GFP:Bsd | GFP-Bsd fusion/fLuc | SB10 DNA |
| D2-NGCD | PBLs | pT2/Cag-NGCDa | NGCD | SB10 DNA |
| PBL15 | PBLs | pKT2/Ubc-19BB_mCMV-QBINGCD | CD19 CAR/NGCD | SB10 DNA |
| UCB15 | UCB | pKT2/Ubc-19BB_mCMV-QBINGCD | CD19 CAR/NGCD | SB10 DNA |
| UCB32 | UCB | pT2/PGK-19BB_mCMV-QBICD20b | CD19 CAR/CD20 | SB10 DNA |
| PBL32-1 | PBLs | pT2/PGK-19BB_mCMV-QBICD20b | CD19 CAR/CD20 | SB10 DNA |
| PBL32-2 | PBLs | pT2/PGK-19BB_mCMV-QBICD20b | CD19 CAR/CD20 | SB10 DNA |
| NGCD-gLuc | PBLs | pT2/EF1-NGCD_mCMV-gLuc | NGCD-gLuc | SB10 DNA |
| PBL9 | PBLs | pKT2/Cag-DsRedc | DsRed | SB11 DNA |
| UCB1 | UCB | pKT2/Cag-DsRedc | DsRed | SB11 DNA |
| PBL1m | PBLs | pKT2/Cag-DsRedc | DsRed | SB11 mRNAd |
| PBL1d | PBLs | pKT2/Cag-DsRedc | DsRed | SB11 DNA |
| PBL2m | PBLs | pKT2/Cag-DsRedc | DsRed | SB11 mRNAd |
| PBL2d | PBLs | pKT2/Cag-DsRedc | DsRed | SB11 DNA |
PBL, peripheral blood lymphocytes; UCB, umbilical cord blood; NGCD, truncated nerve growth factor receptor–cytosine deaminase fusion; EF1, human elongation factor-1α promoter; Cag, chimeric CMV-enhancer chicken β-actin promoter; PGK, human phosphoglycerate kinase promoter; GFP, green fluorescent protein; Zeo, Zeocin resistance gene; Bsd, blasticidin resistance gene; 19BB, CD19 CAR with 4-1BB signaling domain; QBINGCD, QBI translational enhancer element proceeding NGCD; QBICD20, QBI proceeding CD20; fLuc, firefly luciferase; gLuc, gaussia luciferase; CD19 CAR, chimeric antigen receptor for CD19; CD20, CD20 antigen.
Huang et al. (2006).
Huang et al. (2008).
Huang et al. (2010).
Wilber et al. (2006).