Table 2.
Identity of Recovered Plasmids for Cytoplasmic Hirt DNA Preparationsa
| |
|
|
|
Identity of recovered plasmids |
|
|
|---|---|---|---|---|---|---|
| T cells | Genomic PCR for SB10 | Total colonies | Number evaluated | Transposon | Transposase | Unknown |
| PBL pT2/DsRed+pUb-SB10 day 7 | n.d. | 3000 | 20 | 14 | 3 | 3 |
| SB82 | + | 4 | 4 | 0 | 0 | 4 |
| NGCD-gLuc | n.d. | 4 | 4 | 0 | 0 | 4 |
| AP1 | + | 5 | 5 | 0 | 0 | 5 |
| AP2 | + | 0 | 0 | 0 | 0 | 0 |
| PBL32-1 | n.d. | 1 | 1 | 0 | 0 | 1 |
| PBL32-2 | + | 0 | 0 | 0 | 0 | 0 |
| Clone O56 | + | 1 | 1 | 0 | 0 | 1 |
| pUC19 control | 4.65 × 1010 | n.d. | n.d. | n.d. | n.d. | |
n.d., not done.
a
Shown is a representative example of two independent transformations using Hirt DNA.