Table 3.
Characterization of Transposon Integration Events
| T cell clone | Year cultured | Chromosome location: hit from: | Located gene | Gene symbol | Gene orientation | Cancer-related gene | Proximal TSS | Distance to TSS (bp)a | Integration frequency |
|---|---|---|---|---|---|---|---|---|---|
| O69 | 2004 | chr13:55526482 | Intergenic | PRR20A | (+) | Yes | chr13:56613053 | −1086572 | N/A |
| 2006 | chr13:55526482 | Intergenic | PRR20A | (+) | Yes | chr13:56613053 | −1086572 | N/A | |
| 2004 | chr11:34925030 | Intronic | PDHX | (+) | Yes | chr11:34894252 | +30777 | 21/24 | |
| 2004 | chr11:34925570 | Intronic | PDHX | (+) | Yes | chr11:34894252 | +30777 | 3/24 | |
| 2006 | chr11:34925025 | Intronic | PDHX | (+) | Yes | chr11:34894252 | +30777 | 1/24 | |
| O56 | 2006 | chr11:34925030 | Intronic | PDHX | (+) | Yes | chr11:34894252 | +30777 | 20/24 |
| 2006 | chr11:34925570 | Intronic | PDHX | (+) | Yes | chr11:34894252 | +30777 | 2/24 | |
| 2006 | chr11:34925572 | Intronic | PDHX | (+) | Yes | chr11:34894252 | +30777 | 1/24 | |
| 2006 | chr11:34874627 | Intronic | APIP | (–) | No | chr11:34894515 | +20112 | 1/24 |
N/A, not applicable because of pure PCR products, which were not subject to TOPO cloning; TSS, transcriptional start site; PRR20A, proline-rich 20A; PDHX, pyruvate dehydrogenase complex component X; APIP, APAF1-interacting protein.
a
+, intragenic; –, upstream of TSS.