Table 1.
The effect of AG1478 and DAPH on phenylephrine concentration-response curves
| Vehicle | AG1478 (2.5 µM) | AG1478 (5 µM) | AG1478 (10 µM) | AG1478 (20 µM) | |
|---|---|---|---|---|---|
| Endothelium-intact rings | |||||
| Emax (%) | 99.2 ± 3.1 | 96.6 ± 0.4 | 96.3 ± 2.3 | 84.4 ± 3.7a | 76.8 ± 3.6a |
| pEC50 | 7.39 ± 0.05 | 7.16 ± 0.06 | 6.79 ± 0.12a | 6.36 ± 0.08a | 5.99 ± 0.04a |
| Endothelium-denuded rings | |||||
| Emax (%) | 99.1 ± 5.1 | 99.8 ± 2.6 | 92.5 ± 5.1 | 89.0 ± 5.1a | 76.9 ± 9.5a |
| pEC50 | 7.43 ± 0.16 | 7.15 ± 0.13 | 7.01 ± 0.15 | 6.48 ± 0.15a | 6.26 ± 0.06a |
| Vehicle | DAPH (1 µM) | DAPH (5 µM) | DAPH (10 µM) | ||
| Endothelium-denuded rings | |||||
| Emax (%) | 93.1 ± 2.3 | 77.8 ± 13.9 | 70.3 ± 12.5 | 46.8 ± 11.2a | |
| pEC50 | 7.01 ± 0.25 | 6.86 ± 0.25 | 6.60 ± 0.20 | 6.20 ± 0.16a | |
Data are given as mean ± SEM.
a
P < 0.05 versus vehicle.
DAPH, 4,5-dianilinophthalimide, 5,6-bis(phenylamino)-1H-isoindole-1,3(2H)-dione; Emax, maximal contraction response in % of KCl; pEC50, negative logarithm of the EC50.